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ZKO number ZKO1144
Recent progress of this line F2 individuals have been indentified
Gene name si:ch73-343l4.3
Ensembl ID ENSDARG00000092502
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGATGAGCTGGTATGAGAGCagg
Cas9 target site 2 sequence
Position in the gene exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) gctcttctgctgctcgtc
PCR primer (reverse) ctgacttacCCTCGCTTC
Length of expected PCR product (bp) 467
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1144
Dosage of injected Cas9 mRNA (ng/ul) 650
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 90
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1144.pdf
Mutation efficiency in F0 embryos (%) 86
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 14%
Wild-type (WT) sequence AAGAGGATGAGCTGGTATGAGAGCAGGCAGTT(with SNPs)
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 AAGAGGATGAGCTGGTATGAG--CAGGCAGTT
F0-2 AAGAGGATGAGC------------AGGCAGTT
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 AAGAGGATGAGCTGGTATGAG--CAGGCAGTT
F0-2 AAGAGGATGAGC------------AGGCAGTT
Allele name and predicted product 1144a
1144b
ATGAGCTGGTATGAGCAGGCAGTTCTGCAGGAATCATGGAGCTGA truncated protein (16aa)
ATGAGCAGGCAGTTCTGCAGGAATCATGGAGCTGATCTGGTCATTATTAAGAGTGAAGTGAAGCAGAGGTTCATATCCTTATTTGTGAAAGAGAGAGTGTGGATCGGTTTGTCTGACACAGAGACTGAAGGCATCATGAAGTGGGTGGATAATTCAACACTGAAGCGAGGGTTTTGGCAGAAAGAGCTTGATGGTAATGCAGGAAATGAAGACTGTGTTGAACTGAGGAATACATCACCTGCCCTGAACAACTGGAATGATCTGTTATGCTCAGACAATAGAATAGGGGTTTGTGAGAAA delete aa4-7
突变创建时间 2014-12-11
填表时间 2013/12/20
修改时间 2016/8/9
PI/负责人 (工作单位)
Genotype Information:
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