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ZKO number ZKO1141
Recent progress of this line F2 individuals have been indentified
Gene name samd1b
Ensembl ID ENSDARG00000079062
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
TCTACCCGCGAGGGGGATGGggg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) n/a
PCR primer (forward) AATGGATGCTGTCCGAAGTA
PCR primer (reverse) AAGAGCGGAAATCAGAAGGT
Length of expected PCR product (bp) 449
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids?used?for?making?gRNA pT7-gRNA-ZKOpT7-gRNA-ZKO
Primers?used?for?making?gRNA Pr-ZKO 1141
Dosage of injected Cas9 mRNA (ng/ul) 650
Dosage of injected gRNA (ng/ul) 69
Survival rate of F0 embryo @ 1dpf (%) 90
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1141.pdf
Mutation efficiency in F0 embryos (%) 72
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 50%
Wild-type (WT) sequence TCGGCCACATCACTCTACCCGCGAGGGGGATGGGGGCGGCCAAGCC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 TCGGCCACATCACTCTACCCGCGAGGGGGA-------GGCCAAGCC(-7bp)
F0-2 TCGGCCACATCACTCTACCCGCGAGGGG-----------CCAAGCC(-11bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TCGGCCACATCACTCTACCCGCGAGGGGGA-------GGCCAAGCC(-7bp)
F0-2 TCGGCCACATCACTCTACCCGCGAGGGG-----------CCAAGCC(-11bp)
Allele name and predicted product 1141a
1141b
AGCGGATCAC--------AGAGCTGAAGGATGA ; Truncated protein 396aa
AGCGGATCAC-----AGCTTTCTGCTGA; no stop coden
突变创建时间 2014-07-31
填表时间 2013/12/20
修改时间 2016/8/9
PI/负责人 (工作单位)
Genotype Information:
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