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ZKO number ZKO1171
Recent progress of this line F2 individuals have been indentified
Gene name CABZ01059392.1
Ensembl ID ENSDARG00000067957
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGACTGTGTGACACGTCAATcgg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) n/a
PCR primer (forward) 5’- GAAAGAGCGCCACAGGAAAC -3’
PCR primer (reverse) 5’- GCTCACCCCGATTAGCTTCT -3’
Length of expected PCR product (bp) 327
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1171
Dosage of injected Cas9 mRNA (ng/ul) 667
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 90
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1171.pdf
Mutation efficiency in F0 embryos (%) 80
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 46%
Wild-type (WT) sequence GGACTGTGTGACACGTCAATcgg
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 GGACTGTGTGACACGxxxxxxxg (+0 bp, -7 bp) GGACTGTGTGACACGTCxxxxgg (+0 bp, -4bp)
F0-2 GGACTGTGTGACACGxxxxTcgg (+0 bp, -4 bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 10%(2/20: Number of positive F1/ Total number of F1 creened) GGACTGTGTGACAxxxxxxcgg(+0bp, -7bp)
F0-2 GGACTGTGTGACACGTCxxxxgg(+0bp,-4bp)
Allele name and predicted product 1171a
1171b
AAGCTTGATGATTGCACTGATCTCAGGATCGTGATGGTGGGTAAAACCGGAGCCGGAAAG AGCGCCACAGGAAACACCATCCTGAGACAAAAAGTGTTTAAGGAAGAACTATCAGCTAAA TCTGTGACCAAGAAATGCCAGAAACACCAGCGGGAGGTGAGCGGTCGAATCATCTCCATC ATCGACACTCCGGGACTGTGTGACACGTCGGCGAAGAAGACCTCAAGAAAGAAATC GAGAAATGTGTCTACATGTCTGCTCCCGGTCCTCACGTGTTCCTGCTGGTGCTCAGACTGGACAGACTCACAAACGAAGAGAAAAACACTGTGA Truncated protein (110aa)
突变创建时间 2014-09-11
填表时间 2013/12/20
修改时间 2016/8/9
PI/负责人 (工作单位)
Genotype Information:
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