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ZKO number ZKO1169
Recent progress of this line F2 generation has been raised
Gene name CABZ01059391.1
Ensembl ID ENSDARG00000040928
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGTGTCGCAGGTGCTGTGGGagg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) AlwNI (CAGNNNCTG)
PCR primer (forward) 5’- CAGGTGATGGGTGTGGTTCT -3’
PCR primer (reverse) 5’- CTTGAGGCCAGTGATGCAGA -3’
Length of expected PCR product (bp) 288
Length of expected RE digestion product (bp) 117+171
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO
Dosage of injected Cas9 mRNA (ng/ul) 600
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1169.pdf
Mutation efficiency in F0 embryos (%) 88.9
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 100%
Wild-type (WT) sequence GTTCGGGATCGTGGTCTGCATCGCCCCCTGGTGGCGCACGTCTGCATTCGTGGGCGATGAGCTGGTGGTGTCGCAGGTGCTGTGGGAGGGGCTCTGGATGTCCTGTCTGTC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 GTTCGGGATCGTGGTCTGCATCGCCCCCTGGGATCGTGGTGGGAGGGGCTCTGGATGTCCTGTCTGTC
F0-2 CTGGTGGCGCACGTCTGCATTCGTGGGCGACACCACCAGCTCTGGATGTCCTGTCTGTCGGAGCGGGG
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GTTCGGGATCGTGGTCTGCATCGCCCCCTGGGATCGTGGTGGGAGGGGCTCTGGATGTCCTGTCTGTC
F0-2 CTGGTGGCGCACGTCTGCATTCGTGGGCGACACCACCAGCTCTGGATGTCCTGTCTGTCGGAGCGGGG
Allele name and predicted product 1169a
1169b
GTTCGGGATCGTGGTCTGCATCGCCCCCTGGGATCGTGGTGGGAGGGGCTCTGGATGTCCTGTCTGTC
CTGGTGGCGCACGTCTGCATTCGTGGGCGACACCACCAGCTCTGGATGTCCTGTCTGTCGGAGCGGGG
突变创建时间 2013-11-12
填表时间 2013/12/20
修改时间 2016/8/9
PI/负责人 (工作单位)
Genotype Information:
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