Chinese | English
用户名 密码 验证码 验证码

ZKO number ZKO1155
Recent progress of this line F2 generation has been raised
Gene name null
Ensembl ID CABZ01036821.1 ENSDARG00000086938
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
Cas9 target site 2 sequence
Position in the gene Intron1-Exon2
Restriction enzyme (RE) n/a
PCR primer (forward) 5’- TCCCTTAATTCTGATTGGCTG -3’
PCR primer (reverse) 5’- TCCTGAAGACAAGCCTTTCCT -3’
Length of expected PCR product (bp) 400
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pSP6-2sNLS-spCas9
Plasmids?used?for?making?gRNA pT7-gRNA
Primers?used?for?making?gRNA Pr-ZKO
Dosage of injected Cas9 mRNA (ng/ul) 670
Dosage of injected gRNA (ng/ul) 45
Survival rate of F0 embryo @ 1dpf (%) 90
Method to estimate mutation efficiency PCR and single clone sequencing
Stage of the embryos collected for mutation detection
Mutation efficiency in F0 embryos (%) 91.7
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 50%(2/4: Number of positive F0 / Total number of F0 screened)
Wild-type (WT) sequence tgaatgtagcGGTCCTTACCAGTGGATAACtggtggtcaaatc
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 tgaatgtagcGGTCCTTACCAGTGGAggtggtcaAACtggtggtcaaatc (+8 bp, -1 bp) tgaatgtagcGGTCCTTACCAGTGGAxxxxxxxtggtcaaatc (-7 bp)
F0-2 tgaatgtagcGGTCCTTACCAGTGGAccttaccagaccttaccagtccttaccagtgAACtggtggtcaaatc (+31 bp, -1 bp) tgaatgtagcGGTCCTTACCAGTGxxxxxxxxxxxgtcaaatc (-11 bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 78.95% (30/38): Number of F1 carrier / Total number of F1 screened) tgaatgtagcGGTCCTTACCAGTGGATxxxxxxxggtcaaatc (-7 bp) tgaatgtagcGGTCCTTACCAGTGGAggtggtcaAACtggtggtcaaatc (+8 bp, -1 bp) tgaatgtagcGGTCCTTACCAGTGGATggcccttaccaAACtggtggtcaaatc (+11 bp) tgaatgtagcGGTCCTTACCAGTGxxxAACtggtggtcaaatc (-3 bp) tgaatgtagcGGTCCTTACCAGTGGATggtcaaaaccaAACtggtggtcaaatc (+11 bp) tgaatgtagcGGTCCTTACCAGTGGxxxxxxxxxxxtcaaatc (-11 bp)
F0-2 87.5% (7/8): Number of F1 carrier / Total number of F1 screened) tgaatgtagcGGTCCTTACCAGTGGxxAACtggtggtcaaatc (-2 bp) tgaatgtagcGGTCCTTACCAGTGGxxxxxxxxxxxtcaaatc (-11 bp) tgaatgtagcGGTCCTTACCAxxxxxxxACtggtggtcaaatc (-7 bp) tgaatgtagcGGTCCTTACCAGTGGxxxxxxxxtggtcaaatc (-8 bp) tgaatgtagcGGTCCTTACCAGTGGAagtggtggtcaaatc (-4 bp, +2 bp)
Allele name and predicted product 1155a
tgaatgtagcGGTCCTTACCAGTGGATxxxxxxxggtcaaatc; Truncated protein (24 aa)
tgaatgtagcGGTCCTTACCAGTGGAggtggtcaAACtggtggtcaaatc; Truncated protein (27 aa)
突变创建时间 2014-06-04
填表时间 2013/12/20
修改时间 2016/8/9
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:; 邮箱
Copyright © 2012 - 2020 国家斑马鱼资源中心 版权所有
鄂ICP备05003091号-2  鄂公网安备42010602003695