国家水生生物种质资源库国家斑马鱼资源中心 (China Zebrafish Resource Center)

ZKO number		ZKO880
Recent progress of this line		F1 progeny has been raised
Gene name		F8A2
Ensembl ID		ENSDARG00000025518
Zebrafish strain		AB
Cas9 target site 1 sequence		sense strand GATTTTCTGATGCGCTACCGGGCGG
Cas9 target site 2 sequence
Position in the gene		Exon 1
Restriction enzyme (RE)		Nci I(CC︱SGG)
PCR primer (forward)		ATGGGAACAATCGGAGAGCG
PCR primer (reverse)		GGTTGTGTGTTGCTAGCCTTG
Length of expected PCR product (bp)		249
Length of expected RE digestion product (bp)		91+158
Plasmids used for making Cas9 mRNA		pGH-T7-zCAS9
Plasmids?used?for?making?gRNA		pMD19T-gRNA
Primers?used?for?making?gRNA		Pr-ZKO 880
Dosage of injected Cas9 mRNA (ng/ul)		300
Dosage of injected gRNA (ng/ul)		30
Survival rate of F0 embryo @ 1dpf (%)		60
Method to estimate mutation efficiency		Restriction enzyme digestionNci I
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO880.pdf
Mutation efficiency in F0 embryos (%)		6.67
Picture?from?F₀?embryos ? (gel?picture?or?sequencing?picture)		/upload/2014-04-03/635321318557831461.jpg 880
Mutation type		deletion
Germline transmission efficiency in adult F₀		2.08%
Wild-type (WT) sequence		GATTTTCTGATGCGCTACCGGGCGG
Germline mutations in selected F0 (By screening F1 embryos)	F0-1
Germline mutations in selected F0 (By screening F1 embryos)	F0-2
Mosaicism in selected F0 (By screening F1 adults)	F0-1	GATTTTCTGATGCGCTAC-GGGCGG(1bp deletion)
Mosaicism in selected F0 (By screening F1 adults)	F0-2
Allele name and predicted product	880a 880b	Truncated protein (17aa)
Allele name and predicted product	880a 880b
突变创建时间		2014-01-07
填表时间		2013/12/20
修改时间		2016/8/8
PI/负责人 (工作单位)

Genotype Information: