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ZKO number ZKO849
Recent progress of this line F2 individuals have been indentified
Gene name si:dkey-9i23.6
Ensembl ID ENSDARG00000094678
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGATTGTTGAAAGTCCCTTT
Cas9 target site 2 sequence
Position in the gene Exon2
Restriction enzyme (RE) FaqI
PCR primer (forward) CTTCAATACAGCTAGTGCAAGG
PCR primer (reverse) CTCAACAGACAGACCACGT
Length of expected PCR product (bp) 424
Length of expected RE digestion product (bp) 163bp +261bp
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 849
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 500
Survival rate of F0 embryo @ 1dpf (%) 60
Method to estimate mutation efficiency Restriction enzyme digestionFaqI
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO849.pdf
Mutation efficiency in F0 embryos (%) 50
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 12.5
Wild-type (WT) sequence CTGAAGAAACGTCCAAAAGGGACTTTCAACAATCCCAAAACAGATA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 CTGAAGAAACGTCCAAAAGGACTTTCAACAATCCCAAAACAGATA
F0-2 CTGAAGAAACGTCCAAAA--CGAAAAAACGACTTTCAACAATCCCAAAACAGATA
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CTGAAGAAACGTCCAAAAGGACTTTCAACAATCCCAAAACAGATA
F0-2 CTGAAGAAACGTCCAAAA--CGAAAAAACGACTTTCAACAATCCCAAAACAGATA
Allele name and predicted product 849a
849b
(+0,-1)
(+9,-2)
突变创建时间 2014-04-30
填表时间 2013/12/19
修改时间 2016/8/8
PI/负责人 (工作单位)
Genotype Information:
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