Chinese | English
用户名 密码 验证码 验证码
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO716
Recent progress of this line F1 progeny has been raised
Gene name galnt7
Ensembl ID ENSDARG00000057303
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GTCAGGTCTCCTGCCATGGCtgg
Cas9 target site 2 sequence
Position in the gene exon 7
Restriction enzyme (RE) Nco1
PCR primer (forward) cacttgtgtgtgaacaggtctactg
PCR primer (reverse) ACGACTACACATTCGAGCAGGATA
Length of expected PCR product (bp) 495
Length of expected RE digestion product (bp) 200+295
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 716
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 50
Method to estimate mutation efficiency Restriction enzyme digestionNco1
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO716.pdf
Mutation efficiency in F0 embryos (%) 50
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 20
Wild-type (WT) sequence GTGTGTCAGGTCTCCTGCCATGGCTGGAGGGCTGTTTG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 GTGTGTCAGGTCTCCTGCCATTGGCTGGAGGGCTGTTTG
F0-2 GTGTGTCAGGTCTCCTGCCATGACTGGAGGGCTGTTTG
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 acaggtgaatttgtgtgtcagGTCTCCAGGTCTCCTGGCTGGAGGGCTGTTTGCAATTGAGAG
F0-2 acaggtgaatttgtgtgtcagGTCTCCTGCCATGCTGGAGGGCTGTTTGCAATTGAGAG
Allele name and predicted product 716a
716b
acaggtgaatttgtgtgtcagGTCTCCTGCCATGGCTGGAGGGCTGTTTGCAATTGAGAG
acaggtgaatttgtgtgtcagGTCTCCTGCCATGCTGGAGGGCTGTTTGCAATTGAGAG
突变创建时间 2014-09-16
填表时间 2013/12/19
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2020 国家斑马鱼资源中心 版权所有
鄂ICP备05003091号-2  鄂公网安备42010602003695