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ZKO number ZKO747
Recent progress of this line F1 individuals have been identified
Gene name si:ch211-113e8.11
Ensembl ID ENSDARG00000076585
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
ccacctccacctctgggctc
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) BslI
PCR primer (forward) ggcaatagtctctattcac
PCR primer (reverse) gcttgtaaagtgagaggta
Length of expected PCR product (bp) 441
Length of expected RE digestion product (bp) 323+118
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 747
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO747.pdf
Mutation efficiency in F0 embryos (%) 80
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 50%
Wild-type (WT) sequence CCATCGGTCCGTCCTTACCCACAAAAACTCCCACCTCCACCTCTGGGCTCAGGTGGAGCGCTTTCTTCAGCCAGCAGTGT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 CCATCGGTCCGTCCTTACCCACAAAAACTCCCACCTCCACCTCAGGTGGAGCGCTTTCTTCAGCCAGCAGTGT
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CCATCGGTCCGTCCTTACCCACAAAAACTCCCACCTCCACCTCAGGTGGAGCGCTTTCTTCAGCCAGCAGTGT
F0-2
Allele name and predicted product 747a
747b
突变创建时间 2015-12-10
填表时间 2013/12/19
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information:
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