Chinese | English
用户名 密码 验证码 验证码
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO982
Recent progress of this line F2 individuals have been indentified
Gene name im:7139293
Ensembl ID ENSDARG00000075564
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGAATTTCAAGGGCCGTCTGagg
Cas9 target site 2 sequence
Position in the gene Exon14
Restriction enzyme (RE) n/a
PCR primer (forward) 5’-TGTGTGTTGGATCGATTGGG-3’
PCR primer (reverse) 5’-CCATAAGGCGTGTAAAGAAGTTT-3’
Length of expected PCR product (bp) 402
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 982
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 50
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO982.pdf
Mutation efficiency in F0 embryos (%) 30
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 30%
Wild-type (WT) sequence TTTCAAGGGCCGTCTGGGGCAATACAACC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 TTTCAAGGGCC----------TACAACC (-10bp)
F0-2 TTTCAA----------GGGCTACAACC(-10bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TTTCAAGGGCC----------TACAACC (-10bp)
F0-2 TTTCAA----------GGGCTACAACC(-10bp)
Allele name and predicted product 982a
982b
TTTCAAGGGCC----------TACAACC Truncated protein (571aa)
TTTCAA----------GGGCTACAACC Truncated protein (588aa)
突变创建时间 2014-10-10
填表时间 2013/12/19
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2016 国家斑马鱼资源中心 版权所有