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ZKO number ZKO1270
Recent progress of this line F2 individuals have been indentified
Gene name slc27a1b
Ensembl ID ENSDARG00000104949
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
ACTGCAGCCTCGCCAACAtgg
Cas9 target site 2 sequence
Position in the gene exon8
Restriction enzyme (RE) n/a
PCR primer (forward) CAATGGTTTGCGGCCTAACG
PCR primer (reverse) CCTTCCAGTGAAGTGTCCCC
Length of expected PCR product (bp) 187
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1270
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 90
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1270.pdf
Mutation efficiency in F0 embryos (%) 80
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 40%(4/10)
Wild-type (WT) sequence CAACTGCAGCCTCGCCAACATGGACAACAAGGTACA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 2.86%(1/35) CAACTGCAGCCTCG-GACATGGACAACAAGGTACA(-3,+1)
F0-2
Allele name and predicted product 1270a
1270b
CAACTGCAGCCTCG-GACATGGACAACAAGGTACA(-3,+1),truncated protein (430aa)
突变创建时间
填表时间 2013/12/18
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
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