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ZKO number ZKO361
Recent progress of this line F1 individuals have been identified
Gene name phox2ba
Ensembl ID ENSDARG00000075330
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGCTTATGAACGAGGCGTCCagg
Cas9 target site 2 sequence
Position in the gene exon2(phox2ba-001)
Restriction enzyme (RE) n/a
PCR primer (forward) TCTTACCGATCACCCTTTTTATTG
PCR primer (reverse) GGGTCCCCATATTTTGCTGTG
Length of expected PCR product (bp) 384
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 361
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 200
Survival rate of F0 embryo @ 1dpf (%) 70
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO361.pdf
Mutation efficiency in F0 embryos (%) 20
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 20%
Wild-type (WT) sequence ATGGCTTATGAACGAGGCGTCCAGGAGCGGCGCAAACAAAGGCGCGTGCGCA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 ATGGCTTATGAACGAGGCGXXXXXXXXXXGCGCAAACAAAGGCGCGTGCGCA(10bp deletion)
F0-2 ATGGCTTATGAACGAGGCGXXXXXXXXXXXXXCAAACAAGGGCGCGTGCGCA(13bp deletion and 27site A→G)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 ATGGCTTATGAACGAGGCGXXXXXXXXXXGCGCAAACAAAGGCGCGTGCGCA(10bp deletion)
F0-2 ATGGCTTATGAACGAGGCGXXXXXXXXXXXXXCAAACAAGGGCGCGTGCGCA(13bp deletion and 27site A→G)
Allele name and predicted product 361a
361b
ATGGCTTATGAACGAGGCGXXXXXXXXXXGCGCAAACAAAG truncated protein(21AA)
ATGGCTTATGAACGAGGCGXXXXXXXXXXXXXCAAACAAGG truncated protein(20AA)
突变创建时间 2013-12-18
填表时间 2013/12/18
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
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