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ZKO number ZKO261
Recent progress of this line F2 individuals have been indentified
Gene name sorting nexin 8b
Ensembl ID ENSDARG00000078057
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGGTCAGCAAGGCCCTCTGAtgg
Cas9 target site 2 sequence
Position in the gene Exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) CACTTTTGACTGGACCTTTCGT
PCR primer (reverse) GTTTTACACAGCGGACACCCT
Length of expected PCR product (bp) 461
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO261.pdf
Mutation efficiency in F0 embryos (%) 30
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0
Wild-type (WT) sequence tttgctgAAGATGCATCTGGGTCAGCAAGGCCCTCTGATGGATGGGCTCTCCTTAAAAGAGCT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TCTGGGTCAGCAAGGCCC-AGGATGGATGGGCTCTCCTTAAAA (-3,+2)
F0-2 TCTGGGTCAGCAAGGCCCTC--CTGGATGGGCTCTCCTTAAAA (-3,+1)
Allele name and predicted product 261a
261b
TCTGGGTCAGCAAGGCCC-AGGATGGATGGGCTCTCCTTAAAA (-3,+2)14aa
TCTGGGTCAGCAAGGCCCTC--CTGGATGGGCTCTCCTTAAAA (-3,+1)44aa
突变创建时间 2013-05-27
填表时间 2013/12/18
修改时间 2016/10/14
PI/负责人 (工作单位)
Genotype Information:
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