Chinese | English
用户名 密码 验证码 验证码
联系客服
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO628
Recent progress of this line F0 population has been raised
Gene name chmp2bb
Ensembl ID ENSDARG00000002190
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
ggaagttctgcagggtctgca
Cas9 target site 2 sequence
Position in the gene exon 4
Restriction enzyme (RE) n/a
PCR primer (forward) cgcataattgcatgaggagcct
PCR primer (reverse) agcatagtccgtctggttaagc
Length of expected PCR product (bp) 303
Length of expected RE digestion product (bp) 156+147
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 628
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 20
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO628.pdf
Mutation efficiency in F0 embryos (%) 3
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0
Wild-type (WT) sequence AACAAAAAGATGGATCCAAAGAAGACCATGCAGACCCTGCAGAACTTCCAGAAAGAAACCGCAAAGATG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 AACAAAAAGATGGATCCAAAGAAGACCATGCAAGACCCTGCAGAACTTCCAGAAAGAAACCGCAAAGATG(+1bp)
F0-2
Allele name and predicted product 628a
628b
突变创建时间
填表时间 2013/12/18
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2023 国家斑马鱼资源中心 版权所有
鄂ICP备05003091号-2  鄂公网安备42010602003695