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ZKO number ZKO909
Recent progress of this line F2 individuals have been indentified
Gene name ebpl
Ensembl ID ENSDARG00000036636
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGTCAACCGCTGAACATTTAtgg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) T7核酸内切酶
PCR primer (forward) AAAGTGCTTTCACTAGACAG
PCR primer (reverse) TATACCAACAAACACCACT
Length of expected PCR product (bp) 480
Length of expected RE digestion product (bp) 336+144
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO
Dosage of injected Cas9 mRNA (ng/ul) 500
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 83.7
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO909.pdf
Mutation efficiency in F0 embryos (%) 36.87
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 44.4
Wild-type (WT) sequence CTCAAAAATGGGGCCATAAATGTTCAGCGGTTGACC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CTCAAAAATGGGGCCATAA-------GCGGTTGACC(-7bp)
F0-2 CTCAAAAATGGGGCCATAAA ++++TTTATGGCCCCATTTTTGAG(-1bp;+5bp)
Allele name and predicted product 909a
909b
MAEFDQQHDEPALFTKVTVYSLLACVAQFAVGFAFAQKWGHKRLFSG-; Truncated protein (47aa)
MAEFDQQHDEPALFTKVTVYSLLACVAQFAVGFAFAQKWGHSRLTGGSSCGYFTTQ-; Truncated protein (56aa)
突变创建时间 2014-09-04
填表时间 2013/12/17
修改时间 2016/8/12
PI/负责人 (工作单位)
Genotype Information:
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