Chinese | English
用户名 密码 验证码 验证码
联系客服
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO969
Recent progress of this line F2 individuals have been indentified
Gene name gstcd
Ensembl ID ENSDARG00000036494
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GCCAGTTGATGCCCGACCGCAggg
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) Bsl I (CCNN/NNNNNGG)
PCR primer (forward) TGTCCTACAGCGAATGCTC
PCR primer (reverse) ctaagcatttccagcacctgt
Length of expected PCR product (bp) 369
Length of expected RE digestion product (bp) 250 bp +119 bp
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 969
Dosage of injected Cas9 mRNA (ng/ul) 1000
Dosage of injected gRNA (ng/ul) 300
Survival rate of F0 embryo @ 1dpf (%) 52
Method to estimate mutation efficiency Restriction enzyme digestion68
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO969.pdf
Mutation efficiency in F0 embryos (%) 30
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 6/20
Wild-type (WT) sequence CCCGACCGCAGGGACGTGGCGTCACTTTT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 CCCGACC AGGGACGTGGCGTCACTTTT(+0bp,-2bp)
F0-2 CCCG-------CAGGGACGTGGCG (+0bp,-4bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CCCGACC AGGGACGTGGCGTCACTTTT(+0bp,-2bp)
F0-2 CCCG-------CAGGGACGTGGCG (+0bp,-4bp)
Allele name and predicted product 969a
969b
GCCAGTTGATGCCCGACC-- AGGGAC truncated protein (128aa)
CCCG---CAGGGACGTGGCG truncated protein (153aa)
突变创建时间 2015-03-18
填表时间 2013/12/17
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2023 国家斑马鱼资源中心 版权所有
鄂ICP备05003091号-2  鄂公网安备42010602003695