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ZKO number ZKO1077
Recent progress of this line F2 generation has been raised
Gene name RHOU
Ensembl ID ENSDARG00000005626
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGGTAAAGTGTGTGATGGTcgg
Cas9 target site 2 sequence
Position in the gene exon2
Restriction enzyme (RE) n/a
PCR primer (forward) GCTGTATTCTACACACACCA
PCR primer (reverse) AGTGTTGTACTTCGATAGTC
Length of expected PCR product (bp) 442
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1077
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 60
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1077.pdf
Mutation efficiency in F0 embryos (%) 0.15
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 12.5%(1/8)
Wild-type (WT) sequence CCGCCTCCGGTTCCACCGCGAGTAGCTCCACCGCGAGACACGCC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CCGCCTCCGGTT-----------AGCTCCACCGCGAGACACGCC
F0-2 CCGCCTCCGG-------------AGCTCCACCGCGAGACACGCC
Allele name and predicted product 1077a
1077b
CCGCCTCCGGTT-----------AGCTCCACCGCGAGACACGCC Truncated protein(27aa)
CCGCCTCCGG-------------AGCTCCACCGCGAGACACGCC Truncated protein(30aa)
突变创建时间 2014-08-11
填表时间 2013/12/17
修改时间 2016/9/23
PI/负责人 (工作单位)
Genotype Information:
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