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ZKO number ZKO1136
Recent progress of this line F2 individuals have been indentified
Gene name si:ch73-341p18.7
Ensembl ID ENSDARG00000075855
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGAACGTCAAAACTGACCAT
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) n/a
PCR primer (forward) 5’-CTAGGGTCCATACTGATACCTT-3’
PCR primer (reverse) 5’-CAATGCAATACACAGCACAAT-3’
Length of expected PCR product (bp) 379
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1136
Dosage of injected Cas9 mRNA (ng/ul) 100
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 35
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1136.pdf
Mutation efficiency in F0 embryos (%) 70
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 25% (3/12)
Wild-type (WT) sequence ACTGGAACGTCAAAACTGACCATGGGCTTT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 ACTGGAACGTCAAAACTGACT-TGGGCTTT(-1,+ 0)
F0-2 ACTGGAACGTCAAAAA----CGTGGGCTTT (-4,+0)
Allele name and predicted product 1136a
1136b
ACTGGAACGTCAAAACTGACT-TGGGCTTT Truncated protein (19aa)
ACTGGAACGTCAAAAA----CGTGGGCTTT Truncated protein (17aa)
突变创建时间 2013-12-17
填表时间 2014/11/13
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
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