国家水生生物种质资源库国家斑马鱼资源中心 (China Zebrafish Resource Center)

ZKO number		ZKO130
Recent progress of this line		F1 individuals have been identified
Gene name		interferon-induced GTP-binding protein MxB
Ensembl ID		ENSDARG00000087845
Zebrafish strain		AB
Cas9 target site 1 sequence		sense strand GGGAAAGAGTTCGGTTCagg
Cas9 target site 2 sequence
Position in the gene		exon1
Restriction enzyme (RE)		T7
PCR primer (forward)		caggagcatcactgggagacaatta
PCR primer (reverse)		tataattttcccctcaccactgccc
Length of expected PCR product (bp)		327
Length of expected RE digestion product (bp)		277+50
Plasmids used for making Cas9 mRNA		pXT7-hCAS9
Plasmids?used?for?making?gRNA		pMD19T-gRNA
Primers?used?for?making?gRNA		Pr-ZKO 130
Dosage of injected Cas9 mRNA (ng/ul)		200
Dosage of injected gRNA (ng/ul)		20
Survival rate of F0 embryo @ 1dpf (%)		80
Method to estimate mutation efficiency		T7EI detection
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO130.pdf
Mutation efficiency in F0 embryos (%)		50
Picture?from?F₀?embryos ? (gel?picture?or?sequencing?picture)		/upload/2015-12-10/635853536357473163.jpg undefined 2014-1-8 MU WT130
Mutation type		deletion
Germline transmission efficiency in adult F₀		20
Wild-type (WT) sequence		gggaaagagttcggttctggagg
Germline mutations in selected F0 (By screening F1 embryos)	F0-1	gggaaagagttcggtt-tggagg
Germline mutations in selected F0 (By screening F1 embryos)	F0-2	gggaaagagttcgg----ggagg
Mosaicism in selected F0 (By screening F1 adults)	F0-1	gggaaagagttcggtt-tggagg
Mosaicism in selected F0 (By screening F1 adults)	F0-2	gggaaagagttcgg----ggagg
Allele name and predicted product	130a 130b	Truncated protein 77aa
Allele name and predicted product	130a 130b	Truncated protein 76aa
突变创建时间
填表时间		2014/3/28
修改时间		2016/9/23
PI/负责人 (工作单位)

Genotype Information: