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ZKO number ZKO974
Recent progress of this line F2 individuals have been indentified
Gene name dkk2
Ensembl ID ENSDARG00000076420
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGCAGCGCTTCTTCCTCCGGcgg
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) n/a
PCR primer (forward) GTTATCCATGCAGCAGTGATAAAG
PCR primer (reverse) CATAGAGGATTTGTGCGAGGAC
Length of expected PCR product (bp) 299
Length of expected RE digestion product (bp) N/A
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 974
Dosage of injected Cas9 mRNA (ng/ul) 200
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 85
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO974.pdf
Mutation efficiency in F0 embryos (%) 60
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 50% (4/8)
Wild-type (WT) sequence TCCCGCTGCCTGTCATGCCGCCGGAGGAAGAAGCGCTGCC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TCCCGCTGCCTGTCATGCCGCCGGA-GAAGAAGCGCTGCC (-1bp)
F0-2
Allele name and predicted product 974a
974b
TCCCGCTGCCTGTCATGCCGCCGGA-GAAGAAGCGCTGCC Truncated protein (126aa)
突变创建时间
填表时间 2014/1/2
修改时间 2016/11/11
PI/负责人 (工作单位)
Genotype Information:
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