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ZKO number ZKO1139
Recent progress of this line F2 individuals have been indentified
Gene name zgc:77820
Ensembl ID ENSDARG00000033789
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGATGGGCGCTCATCTTGTGcgg
Cas9 target site 2 sequence
Position in the gene Exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) TTCCTGCGCAGATCGGAAAC
PCR primer (reverse) TACCGTGTATTTGAGCAGT
Length of expected PCR product (bp) 311
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1139
Dosage of injected Cas9 mRNA (ng/ul) 100
Dosage of injected gRNA (ng/ul) 150
Survival rate of F0 embryo @ 1dpf (%) 50
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1139.pdf
Mutation efficiency in F0 embryos (%) 10
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 33% (2/6)
Wild-type (WT) sequence ATCGGGATGGGCGCTCATCTTGTGCGGA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 ATCGGGATGGGCGCTCA-----TGCGGA
F0-2
Allele name and predicted product 1139a
1139b
ATCGGGATGGGCGCTCA-----TGCGGA Truncated protein(11aa)
突变创建时间 2014-05-21
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
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