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ZKO number ZKO1127
Recent progress of this line F2 generation has been raised
Gene name null
Ensembl ID ENSDARG00000086166
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
cccTTATGGTCTCACTGTGAC
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) n/a
PCR primer (forward) ACTCAAATGGACCATGTGTGTTACC
PCR primer (reverse) AGCTGTAGTTTCCAGAATCTTCCC
Length of expected PCR product (bp) 682
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1127
Dosage of injected Cas9 mRNA (ng/ul) 200
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 56
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1127.pdf
Mutation efficiency in F0 embryos (%) 10
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 25%(1/4)
Wild-type (WT) sequence ACATTTATCAGGCAGTTATACACACCCTTATGGTCTCACTGTGACAAAGATATTTTGGACTGTAAACCCAAAAATAGGAGAATCCATCAATCTGCTGGATA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 ACATTTATCAGGCAGTTATACACACCCTTAAAATAGGAGAATCCATCAATCTGCTGGATA
F0-2
Allele name and predicted product 1127a
1127b
ACATTTATCAGGCAGTTATACACACCCTTAAAATAGGAGAATCCATCAATCTGCTGGATA Truncated protein (26aa)
突变创建时间 2013-12-28
填表时间 2013/12/20
修改时间 2016/8/18
PI/负责人 (工作单位) 石德利(山东大学生命科学学院)
Genotype Information:
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