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ZKO number ZKO1138
Recent progress of this line F2 generation has been raised
Gene name tecrb
Ensembl ID ENSDARG00000025904
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGGTGCATAAATGAAGGGCACT / GAGCGGGTGGTTGATATAAT
Cas9 target site 2 sequence
Position in the gene Exon 7 / Exon 9
Restriction enzyme (RE) n/a
PCR primer (forward) 5’-AGAATTGCAACGGCAGTGAGC-3’ /5’-AAACAAATAAGACTTTCTCCAGAAG-3’
PCR primer (reverse) 5’-GTGGGTGCTTACTTCTGAGTCTACA-3’ / 5’-TCTCTAGGGAAATGCTATCTTGC-3’
Length of expected PCR product (bp) 423
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1138
Dosage of injected Cas9 mRNA (ng/ul) 150
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 25
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1138.pdf
Mutation efficiency in F0 embryos (%) 10
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 29% (2/7)
Wild-type (WT) sequence CTTCCGAGTGCCCTTCATTT/ TGTAGAGCGGGTGGTTGATATAATAGGCCATCCAAGCTGC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CTTCCGAGTAATGCACCCAAATACGACTTCATTT(-4,+18)
F0-2 TGTAAAGCGGGTGGTTGATAGGCGGTGTGTAAAGCGGGTGGTTGATAGAGCCTCCAAGACTGC(-16,+39)
Allele name and predicted product 1138a
1138b
CTTCCGAGTAATGCACCCAAATACGACTTCATTT Truncated protein (109aa)
TGTAAAGCGGGTGGTTGATAGGCGGTGTGTAAAGCGGGTGGTTGATAGACCAT Truncated protein(172aa)
突变创建时间 2013-09-16
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
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