Chinese | English
用户名 密码 验证码 验证码
联系客服
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO1137
Recent progress of this line F2 individuals have been indentified
Gene name dnajb1b
Ensembl ID ENSDARG00000041394
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGAAAAGTTCAAGGAGATCG
Cas9 target site 2 sequence
Position in the gene Exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) 5’-GGCAGGAGCATAAAAGACCG-3’
PCR primer (reverse) 5’-AGAAAACAAACACGAACACAATAGT-3’
Length of expected PCR product (bp) 515
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1137
Dosage of injected Cas9 mRNA (ng/ul) 150
Dosage of injected gRNA (ng/ul) 130
Survival rate of F0 embryo @ 1dpf (%) 65
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1137.pdf
Mutation efficiency in F0 embryos (%) 10
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 50% (4/8)
Wild-type (WT) sequence CGAGGAAAAGTTCAAGGAGATCGCGGAAGC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CGAGGAAAAGTTCAAGGAGA-------AGC ( -7,+0)
F0-2 CGAGGAAAAGTTCAAGGAGA--------GC(-8, +0)
Allele name and predicted product 1137a
1137b
CGAGGAAAAGTTCAAGGAGA-------AGC Truncated protein (52 aa)
CGAGGAAAAGTTCAAGGAGA--------GC Truncated protein (53 aa)
突变创建时间 2013-10-10
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2023 国家斑马鱼资源中心 版权所有
鄂ICP备05003091号-2  鄂公网安备42010602003695