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ZKO number ZKO1121
Recent progress of this line F2 individuals have been indentified
Gene name mri1
Ensembl ID ENSDARG00000075754
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGATCTGCAGAGACCCGGAC
Cas9 target site 2 sequence
Position in the gene Exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) TGAATCGCCGTATTGTCGCTTAT
PCR primer (reverse) TCACTTTAGATCAAGCGCAGGTT
Length of expected PCR product (bp) 610
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 1121
Dosage of injected Cas9 mRNA (ng/ul) 330
Dosage of injected gRNA (ng/ul) 150
Survival rate of F0 embryo @ 1dpf (%) 73.5
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1121.pdf
Mutation efficiency in F0 embryos (%) 50
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type deletion
Germline transmission efficiency in adult F0 42.9%(3/7)
Wild-type (WT) sequence GGGTCATGACGCTGGAAGCGATCCGGTACCGGTCCGGGTCTCTGCAGATCCTCAACCAGCTGCTGCTGCCGCGGGAAACCGTGTACGACG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GGGTCATGACGCTGGAAGCGATCCGGTACCG----------------------------------------CGGGAAACCGTGTACGACG
F0-2 GGGTCATGACGCTGGAAGCGATCCGGTACCG-------TCTCTGCAGATCCTCAACCAGCTGCTGCTGCCGCGGGAAACCGTGTACGACG
Allele name and predicted product 1121a
1121b
GGGTCATGACGCTGGAAGCGATCCGGTACCG----------------------------------------CGGGAAACCGTGTACGACG Truncated protein (29aa)
GGGTCATGACGCTGGAAGCGATCCGGTACCG-------TCTCTGCAGATCCTCAACCAGCTGCTGCTGCCGCGGGAAACCGTGTACGACG Truncated protein (40aa)
突变创建时间 2013-11-17
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information:
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