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CZ1281

作者:zhangyun 发布时间:2020/7/10 17:43:21

arihb1226/+CZRC catalog IDCZ1281

Nature of the mutation

2bp deletedBetween 156bp to 158bp of the wild-type ar coding sequence, GA is deleted in exon4.

Sense Strand Sequence

GGACAGATGAAAGGTCCGGATGAGGTCGGAGCAGTTCAGGGCCCGTCGGAGACTGTCCAGTGCTTGTCGCCCAAGCCCAACCTGACTTTCCACTCGCAGTTGATCTTCCTGAACATTCTGGAGGCCATTGAGCCCGAGGTGGTGAACGCCGGCCATGATCATGGTCAGCCTGACTCAGCGGCTGCTCTTCTCACCAGCCTTAACGAGTTAGG

Uppercase:Exon/coding sequence

Lowercase:intron/noncoding sequence

atcg:Forward/Reverse primer

atcg:Crisper/cas9 target site

Genotyping assay

Primers:

CZ1242_forward:5’- GGACAGATGAAAGGTCCGGATG-3’

CZ1242_reverse:5’- CCTAACTCGTTAAGGCTGGTG-3’

PCR program:

   

Product size: 212p

The sequencing results

Reference

Yu, G., Zhang, D., Liu, W., Wang, J., Liu, X., Zhou, C., Gui, J., Xiao, W. (2018) Zebrafish androgen receptor is required for spermatogenesis and maintenance of ovarian function. Oncotarget. 9:24320-24334

 Availability: Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。


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