CZ1242

nsun2^ihb340/+（CZRC catalog ID：CZ1242）

Nature of the mutation

1bp deleted and 9bp insertion，Between 100bp to 101bp of the wild-type nsun2 coding sequence, A is mutated into GCATTTATG in exon3.

Sense Strand Sequence

ggcaaggttgcaaggaatcaatacaattaatgtatttcaagctcattgcaaaagcacaaggctgtatatattgatgaccttaattgctttttcaggggatttagttttcataattatgaccttttattctttcatcctctaatcagTCATGCCAAAGAAATCCTTCATACCCTTAAAGAGAAATACTTCAAGGAGGTTCAAGATCTTGAGGTGGACGGGCAGAAAATTGAGGCTCCTCAGCCATTAAGTTGgtaggactagttaaaaaatgtggttattatttttgaatatccatgcaaatctgtgttacaaagtacttcagacttgagctttagcctttccatgtcattgtagGTATCCTGATGAACTGGCATGGCATACAAATCTGAGCAGAAAGATCCTCCGCAAATCTCCCCTTCTGGAGAAATTCCACCAGTTCTTGGTCAGCGAAACTGAATCGgtaaaaaaaaacattgttgtcttttgtcactctctgttccgcttacac

Uppercase:Exon/coding sequence

Lowercase:intron/noncoding sequence

atcg:Forward/Reverse primer

atcg:Crisper/cas9 target site

Genotyping assay

Primers:

CZ1242_forward:5’- GGCAAGGTTGCAAGGAAT-3’

CZ1242_reverse:5’- GTGTAAGCGGAACAGAGAGT-3’

PCR program:

Product size: 508p

The sequencing results :

Reference：

None

Availability: Frozen sperm

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