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CZ1242

作者:zhangyun 发布时间:2020/7/10 17:36:49

nsun2ihb340/+CZRC catalog IDCZ1242

Nature of the mutation

1bp deleted and 9bp insertionBetween 100bp to 101bp of the wild-type nsun2 coding sequence, A is mutated into GCATTTATG in exon3.

Sense Strand Sequence

ggcaaggttgcaaggaatcaatacaattaatgtatttcaagctcattgcaaaagcacaaggctgtatatattgatgaccttaattgctttttcaggggatttagttttcataattatgaccttttattctttcatcctctaatcagTCATGCCAAAGAAATCCTTCATACCCTTAAAGAGAAATACTTCAAGGAGGTTCAAGATCTTGAGGTGGACGGGCAGAAAATTGAGGCTCCTCAGCCATTAAGTTGgtaggactagttaaaaaatgtggttattatttttgaatatccatgcaaatctgtgttacaaagtacttcagacttgagctttagcctttccatgtcattgtagGTATCCTGATGAACTGGCATGGCATACAAATCTGAGCAGAAAGATCCTCCGCAAATCTCCCCTTCTGGAGAAATTCCACCAGTTCTTGGTCAGCGAAACTGAATCGgtaaaaaaaaacattgttgtcttttgtcactctctgttccgcttacac

Uppercase:Exon/coding sequence

Lowercase:intron/noncoding sequence

atcg:Forward/Reverse primer

atcg:Crisper/cas9 target site

Genotyping assay

Primers:

CZ1242_forward:5’- GGCAAGGTTGCAAGGAAT-3’

CZ1242_reverse:5’- GTGTAAGCGGAACAGAGAGT-3’

PCR program:

   

Product size: 508p

The sequencing results :

Reference

None

Availability: Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。


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