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CZ1228

作者:zhangyun 发布时间:2020/7/10 17:24:13

hwatsu32c/+CZRC catalog IDCZ1228

Nature of the mutation

The hwa mutant line hwatsu32c, in which exon 1 of the hwa locus harbors a 33bp insertion, a 1bp deletion, and a 1bp substitution allowing a premature stop codon to appear at the 36th codon. Mhwatsu32c mutant embryos at 24 hpf exhibited a calabash- or onion-like shape without the major body axis in addition to alterations of the dorsal and ventral marker

expression pattern.

Sense Strand Sequence

cacaccgaagacaccctgataaatgaggtaagaacctaaaattaaaactgttgtaaactcacgcggtgaaaagccaggaatctctccacgctcctgccaGAAATTAATTGATGTAAATGGAGCGCAGCTGCGACTCAACTGTCGACTTTGCTCAGTTTTAGTGATTTTATACTTACTATTTTACATAAACTAACATTAATAACCACGGGTGAAAATAAAGCAGACGTCAAAATCATTGTTTTATCTTTGATTTGTGATGAAATGTCGCAGCTGGGATCGGCTGTGCCCTCATCAAACTTACCTGAGGGCCTCCCGGTGTCCAGTTTGGCGCTGCTGATCCTGGTGCTCATCCCATGCGTGCTGCTGCTCCTGCTGCTAAATTGTTTGTTTGTGGGCTACAAACTGTTCCGGATGACCCGGAGAAAGAGAGACCGATACGGGTCCGAGATGTCGCTCATGCACTCTTCATATTCCACCCGCCAGAGG

Uppercase:Exon/coding sequence

Lowercase:intron/noncoding sequence

atcg:Forward/Reverse primer

atcg:Crisper/cas9 target site

Genotyping assay

Primers:

CZ1228_forward:5’- CACACCGAAGACACCCTGAT-3’

CZ1228_reverse:5’- CCTCTGGCGGGTGGAATATG-3’

PCR program:

   

Product size: 486p

The sequencing results

 

Reference

Yan, L., Chen, J., Zhu, X., Sun, J., Wu, X., Shen, W., Zhang, W., Tao, Q., Meng, A. (2018) Maternal Huluwa dictates the embryonic body axis through β-catenin in vertebrates. Science (New York, N.Y.). 362(6417)

Availability: Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。


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