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CZ1227

作者:zhangyun 发布时间:2020/7/10 17:17:58

hwatsu01sm/+CZRC catalog IDCZ1227

Nature of the mutation

The hwa mutant line Mhwatsu01sm, the uncharacterized locus si:dkey-121h17.7 on chromosome 21 carries a 7313bp insertion element at the first exon. Mhwatsu01sm mutant embryos form the body axis or are dorsalized by hwa overexpression. hwatsu01sm/tsu01sm zygotic mutants from hwatsu01sm heterozygous fish crosses undergo normal development and grow to adulthood. When Zhwa mutant adult females mate with wild-type or heterozygous male fish, all resultant embryos develop normally during cleavage and blastula stages but lack the embryonic shield at the shield stage.

Genotyping assay

Primers:

CZ1227_F1:5’- TAGCCAACACAAGTCCTCAT-3’

CZ1227_F2:5’- CGTGCAATCGAGCGAACTTT-3’

CZ1227_R :5’- CCAGCTGCGACATTTCATCACAA -3’

The PCR results

-/-

+/-

+/+

CZ1227-F1/R

——

382bp

382bp

CZ1227-F2/R

497bp

497bp

——


Fig. Calabash-like shape with the yolk splitting into two parts and a tail at the vegetal pole

Reference

Yan, L., Chen, J., Zhu, X., Sun, J., Wu, X., Shen, W., Zhang, W., Tao, Q., Meng, A. (2018) Maternal Huluwa dictates the embryonic body axis through β-catenin in vertebrates. Science (New York, N.Y.). 362(6417)

Availability: Embryos/Adults

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。

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