CZ1211

ret^ihb310/+（CZRC catalog ID：CZ1211）

Nature of the mutation

79bp to 87 bp of the wild-type ret exon4 coding sequence, AGTGTATC, is mutated into T.

Sense Strand Sequence

GATCTTCTGTACCGTTTGCGGTCGACGACTCCACTTCTGAACTAGTGGTCACAGCACAAGTGGACCGCGAGGAGAAGGAAGTGTATCATCTGGATATAGTCTGTATGGTACGAACAGAAAGAAATCTGGAGGAGGTCTTCAGATCTTTACATGTGAACATCTATGATGAAGATGATAATTCTCCTTATGTCAACGGGACTGACACTGAAGACGTCTTAGTAGAGTTTGACCGCAGT

Uppercase:Exon/coding sequence

Lowercase:intron/noncoding sequence

atcg:Forward/Reverse primer

atcg:Crisper/cas9 target site

Genotyping assay

Primers:

CZ1211_forward:5’- GATCTTCTGTACCGTTTGCG-3’

CZ1211_reverse:5’- ACTGCGGTCAAACTCTAC-3’

PCR program:

Product size: 236p

The sequencing results :

Reference：

None

Availability:  Frozen sperm

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