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CZ1340

作者:zhangyun 发布时间:2020/6/23 3:12:00

kcnj2b ihb388/+ (CZRC catalog ID: CZ1340) 

Nature of the mutation

Between 23 bp to 26 bp of the wild-type kcnj2b coding sequence, GATA is mutanted to ATAGGGGGCACT in exon 1.

Sense Strand Sequence

ctaaagcagacgaggctcagccccctcatacggaagcgATGGGAAGCGTGAGAGCCCACCGATACAGCATAGTTTCCTCAGAAGAAGGTGGGATGAAGTTGTCCCCCATTGCTATTCAAAACGGCTACGGCAATGGAAAAGTGAGCACCACAGATCACGAGCAACAGCAAACGCAAAACCGATTCGTCAACAAGGATGGACGCTGCAATGTACAGTTCATCAACATGAGCGAAAAAGGTCAGCGATACCTAGCTGATATCTTCACAACATGCGTTGACATTCGCTGGCGTTGGATGATGGTCCTTTTTTTCCTGTCCTTCCTATTGTCTTG

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg : Crispr/cas9 target site

Genotyping assay

Primers:

kcnj2b _forward: 5'  CTAAAGCAGACGAGGCTCAG  3'

kcnj2b _reverse: 5'  CAAGACAATAGGAAGGACAG  3'

PCR program:

 

Product size: 331 bp

The sequencing results of the CZ1340:

 

Reference:

None

Availability:  Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。


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