CZ1339

bicd2 ^ihb387/+ (CZRC catalog ID: CZ1339) 

Nature of the mutation

In 269 bp of the wild-type bicd2 coding sequence, G is inserted in exon 2.

Sense Strand Sequence

ccactcgtatgctgtaatcataattgtaaatttaattgtaaatttgtttctgaagtgtctgaataacatttggtttgactgtacattccataacatccatttagtagtgttgtacaagtgctttaaacttcaaacctccacccccatccatcctccaccctacaagacaagtaattagcttgtaaaccacagagctagatcttactagactttttacagaatttaaccctcaatctctctcctatcatgttcccacagGCCTATGGCCAGGTTCACTCAACCCACAGAAAGGTGGCTGCGGACGGCGAGAGCAGGGAGGAGTCGCTCATCCTGGAGTCGGCATCTAAAGAGGCGTACTATGAGCAGAGGGTGCAGGAGCTGCAGTCGGACCTACGACAAGCTAAAAACACCCTCACCGGCACCCAGGCGGAGAACGAGCG

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg: Crispr/cas9 target site

Genotyping assay

Primers:

bicd2 _forward: 5'  CCACTCGTATGCTGTAATCA  3'

bicd2_reverse: 5'  CGCTCGTTCTCCGCCTGGGT  3'

PCR program:

Product size: 440 bp

The sequencing results of the CZ1339:

Reference:

None

Availability:  Frozen sperm

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