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CZ1338

作者:zhangyun 发布时间:2020/6/23 3:57:00

bicd2 ihb386/+ (CZRC catalog ID: CZ1338) 

Nature of the mutation

Between 268 bp to 271 bp of the wild-type bicd2 coding sequence, AGAA is deleted in exon 2.

Sense Strand Sequence

ccactcgtatgctgtaatcataattgtaaatttaattgtaaatttgtttctgaagtgtctgaataacatttggtttgactgtacattccataacatccatttagtagtgttgtacaagtgctttaaacttcaaacctccacccccatccatcctccaccctacaagacaagtaattagcttgtaaaccacagagctagatcttactagactttttacagaatttaaccctcaatctctctcctatcatgttcccacagGCCTATGGCCAGGTTCACTCAACCCACAGAAAGGTGGCTGCGGACGGCGAGAGCAGGGAGGAGTCGCTCATCCTGGAGTCGGCATCTAAAGAGGCGTACTATGAGCAGAGGGTGCAGGAGCTGCAGTCGGACCTACGACAAGCTAAAAACACCCTCACCGGCACCCAGGCGGAGAACGAGCG

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg: Crispr/cas9 target site

Genotyping assay

Primers:

bicd2 _forward: 5'  CCACTCGTATGCTGTAATCA  3'

bicd2_reverse: 5'  CGCTCGTTCTCCGCCTGGGT  3'

PCR program:

  

Product size: 440 bp

The sequencing results of the CZ1338:


Reference:

None

Availability:  Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。

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