CZ1337

kras ^ihb385/+ (CZRC catalog ID: CZ1337) 

Nature of the mutation

Between 150 bp to 157 bp of the wild-type kras coding sequence, GTGTCTA is mutanted to ATC in exon 3.

Sense Strand Sequence

gcagatgaacttgttagggcactcaacattcatggtgtcaagaccagacctaaataaactaatcggatgaatattgtacaaggatctatgctaaatgcgcttgtttgtgcatacatgtgatggtgtttttattcagctcaaagaatataattttggctgatgcagGACTCGTACAGGAAGCAGGTGGTGATTGACGGAGAGACGTGTCTACTGGACATCCTGGACACTGCAGGTCAGGAGGAGTACAGTGCCATGAGGGACCAGTACATGAGGACAGGAGAGGGCTTCCTCTGTGTCTTTGCCATCAATAACACCAAGTCCTTCGAGGACATTCACCACTACAGgtgagggtttgctagaaacagatggagtgcagtctcgaaactttcagaaacgaggctgtgaaaagtagagaacgacagggcataact

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg : Crispr/cas9 target site

Genotyping assay

Primers:

kras _forward: 5'  GCAGATGAACTTGTTAGGGC  3'

kras _reverse: 5'  AGTTATGCCCTGTCGTTCTC  3'

PCR program:

Product size: 431 bp

The sequencing results of the CZ1337:

Reference:

None

Availability:  Frozen sperm

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