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CZ1296

作者:zhangyun 发布时间:2020/6/23 11:00:00

gper1 ihb374/+ (CZRC catalog ID: CZ1296) 

Nature of the mutation

Between 269 bp to 275 bp of the wild-type gper1 coding sequence, ATCTTAT was inserted in exon 2.

Sense Strand Sequence

TCAGATTTATGTGAATGGCACCGAGCAGTTCAATGCTTCGTTTGACTTCAACATAACTGATGTGAAAGAAAGCACAGACACCTATGAGTTTTACATCATCGGCCTGTTTCTCTCATGCCTGTACACCATTTTCCTTTTCCCAATTGGTTTCATTGGGAACATCCTCATTTTGGTGGTCAACCTCAACCACAGGGAGAGGATGACCATCCCAGATCTGTACTTTGTCAACCTGGCTGTGGCAGATCTTATTCTGGTGGCAGACTCTCTCATTGAAGTCTTCAATCTCAACGAGAAGTACTACGACTATGCTGTACTGTGTACCTTCATGTCGCTTTTTCTCCAGGTAAACATGTATAGCAGCATCTTCTTCCTGACATGGATGAGTTTCGACCGCTACGTTGCTCTCACCAGCTCTATGAGCAGCAGTCCATTGCGAACCA

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg : Crispr/cas9 target site

Genotyping assay

Primers:

CZ1296_forward: 5'  TCAGATTTATGTGAATGGCA  3'

CZ1296_reverse: 5'  TGGTTCGCAATGGACT  3'

PCR program:

 

Product size: 440 bp

The sequencing results of the CZ1296:


Reference:

None

Availability:  Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。

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