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CZ1288

作者:zhangyun 发布时间:2020/6/23 11:40:00

kcnj2a ihb500/+ (CZRC catalog ID: CZ1288) 

Nature of the mutation

Between 226 bp to 227 bp of the wild-type kcnj2a coding sequence, TG was deleted in exon 1.

Sense Strand Sequence

GAAACAGCACAGAACTCCCAGACTGAGGTGATGGGAAGTGTGCGGGCCAACCGCTACAGCATTGTGTCATCAGAGGAGGACGGTATGAAGTTGGCCACTGCTGCGGTGCCAAATGGGTACGGTAACGGGAAGAGCAAAGTGCACACCCGTCACCAAACCCAAAGCAGGTTCGTCAAGAAAGACGGACACTGCAACGTGCAGTTCATCAATGTCAGCGAAAAGAGCCAGCGCTACCTGGCTGACATCTTCACCACATGCGTGGACATCCGCTGGCGATGGATGTTCGTTATCTTTTGCTTGGCCTTCCTGCTGTCGTGGCTGTTTTTTGGATGTATCTTCTGGTTGGTTGCCATATTCCATGGAGACCTGGAGAACAATGGTCACAA

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg : Crispr/cas9 target site

AAGAGCCAGCGCTACCTGGCTGACATCTTCACCACA

Genotyping assay

Primers:

CZ1288_forward: 5'  GAAACAGCACAGAACTCCCA  3'

CZ1288_reverse: 5'  GAAACAGCACAGAACTCCCA  3'

PCR program:

  

Product size: 386 bp

The sequencing results of the CZ1288:


Reference:

None

Availability:  Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。


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