Chinese | English
用户名 密码 验证码 验证码
联系客服
当前位置:首页 > 详细信息

CZ1286

作者:zhangyun 发布时间:2020/6/22 5:00:00

braf ihb498/+ (CZRC catalog ID: CZ1286) 

Nature of the mutation

Between 846 bp to 855 bp of the wild-type braf coding sequence, CAGCACGGAG was deleted in exon 6.

Sense Strand Sequence

tgaaaaggaccacaaaactaaatatgaaatgaccaaaaagaatgaaattttgccttttggaagcatccaaagcattatttttgttactcgacagGTGCGAAAGACCTTCTTCACGCTGGCCTTCTGTGATTTTTGCCGGAAGCTGTTGTTTCAAGGCTTCCGCTGTCAGACGTGTGGCTATAAGTTTCACCAGCGCTGCAGCACGGAGGTCCCACTCATGTGTGTCAACTACGATCAGCTGGAgtgagtgctcatgggagatctttcccttcttctgaaaaatgctcagtaaaaaaggcttggaaataaaacaattaattaatttactctagttgatattcaccatcaaagaaattaagtttcagtcatttatttgtgctttttgtcatt

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg : Crispr/cas9 target site

Genotyping assay

Primers:

CZ1286_forward: 5'  TGAAAAGGACCACAAAACTA  3'

CZ1286_reverse: 5'  AATGACAAAAAGCACAAATA  3'

PCR program:

 

Product size: 390 bp

The sequencing results of the CZ1286:

Reference:

None

Availability:  Frozen sperm

备注:由于系统更新,订购本品系时,请订购CZ500,并备注本CZ号。订购时请务必注意品系的状态并按实际状态订购。


地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2023 国家斑马鱼资源中心 版权所有
鄂ICP备05003091号-2  鄂公网安备42010602003695