CZ416

nr2e3 ^hzu9/+ (CZRC catalog ID: CZ416) 

Nature of the mutation

Between 485 bp to 521 bp of the wild-type nr2e3 coding sequence, 37 bp sequences

were deleted in exon 4. The mutated nr2e3 codes for a truncated protein containing 180 aa, of which 426 aa are identical to wildtype nr2e3.

Sense Strand Sequence

tagtttctgcccttattgtcttcactttcttaaccgttccgttcaatcttctcagCTGTTCAGAATGAACGTCAGCCCCGCAGCACTGCACAAGTCAGATTAGATGCATTGGATGTGGACAAGGACAAGGAGCACCTCGCCACAACCTTGGAGCCCACCTCCTCATCCACCTGCTCTGTCATCAGCCGCCCACTGCTGGGCTCCTCCATCAGCTCCAGCATCAGCTCCACAGATGGTAGCCAACACTCCAACAGTCCCAAAAATGGACACCGCTTTATGGCCAGTCTGATGACAGCTGAGACTTGCGCAAAGCTGGAACCAGAGGATGgtgaggaacatgaccattggtcaaccagcattaccagcaaagttttgttagtaaaaatgagttataatgagttatatctttacaattagtgtaatttcagcaactatgaattacactaaccttaaaccggaacaacattccaatcagacaag

Uppercase: Exon/coding sequence

Lowercase: intron/noncoding sequence

atcg: Forward/Reverse primer

atcg : Crispr/cas9 target site

Genotyping assay

Primers:

CZ416_forward: 5'  TAGTTTCTGCCCTTATTGTCTT  3'

CZ416_reverse: 5'  CTTGTCTGATTGGAATGTTGTT  3'

PCR program:

Product size: 480 bp

The sequencing results of the CZ416:

Reference:

Xie S, Han S, Qu Z, Liu F, et al. Knockout of Nr2e3 prevents rod photoreceptor differentiation and leads to selective L-/M-cone photoreceptor degeneration in zebrafish. Biochim Biophys Acta Mol Basis Dis. 2019 Jun 1;1865(6):1273-1283.

Availability:  Frozen sperm

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